Journal: Cell reports

Article Title: Proteomic screen reveals diverse protein transport between connected neurons in the visual system

doi: 10.1016/j.celrep.2021.110287

Figure Lengend Snippet: KEY RESOURCES TABLE

Article Snippet: Xenopus laevis tadpoles (stage 47/48) with transgene expressing Cre reporter LCMV:ECFP(loxP)EYFP (Y2 line) , National Xenopus Resource Center, MBL , IACUC protocol 08-0083.

Techniques: Virus, Transgenic Assay, Recombinant, Fluorescence, Quantitation Assay, Plasmid Preparation, Avidin-Biotin Assay, Labeling, Expressing, Software

Journal: Frontiers in Neuroanatomy

Article Title: A Quantitative Analysis of the Distribution of CRH Neurons in Whole Mouse Brain

doi: 10.3389/fnana.2017.00063

Figure Lengend Snippet: Specific EYFP-expression of CRH-IRES-Cre;Ai3 mouse brain. (A–J) Representative photomicrographs depicting CRH neurons expressing EYFP (green), CRH immunostaining (red) and their merged images in MOB, CEA, PVH, B, ECU, IO, MPN, PVT, PG, and TRN, respectively, in a CRH-IRES-Cre;Ai3 mouse brain. The arrowheads show EYFP and CRH double-positive neurons. Scale bar: 40 μm. Abbreviations: main olfactory bulb (MOB), central amygdalar nucleus (CEA), paraventricular hypothalamic nucleus (PVH), Barrington’s nucleus (B), external cuneate nucleus (ECU), inferior olivary complex (IO), medial preoptic nucleus (MPN), paraventricular nucleus of the thalamus (PVT), pontine gray (PG), and tegmental reticular nucleus (TRN).

Article Snippet: CRH-Cre transgenic mice ( ) and EYFP Cre reporter Ai3 mice ( ) were obtained from the Josh Huang Lab (CSHL, Cold Spring Harbor, NY, United States) and Jackson Laboratories (JAX Mice stock number: 007903), respectively.

Techniques: Expressing, Immunostaining

Journal: Frontiers in Neuroanatomy

Article Title: A Quantitative Analysis of the Distribution of CRH Neurons in Whole Mouse Brain

doi: 10.3389/fnana.2017.00063

Figure Lengend Snippet: The brain-wide distribution of CRH neurons imaged by BPS. (A) Three-dimensional rendering of EYFP-labeled CRH neurons. Resampling at 2 μm × 2 μm × 2 μm. (B) Sagittal reconstruction of the maximum intensity projection at the location indicated by a dot dash line in (A) in the CRH-IRES-Cre;Ai3 mouse brain. Projection thickness was 100 μm. (C) The 50-μm-thick maximum intensity projections of the coronal slices indicated by dash lines in (B) , respectively. (D) Enlarged views of EYFP-labeled CRH neurons of the area indicated with text annotations and white boxes in (C). Images of MOs and HPF were the maximum intensity projections of 50 μm, reflecting the sparse distribution of CRH neurons. Images of PIR, ACB, PVH, B, and IO were original data of a single slice. (E) Original images of EYFP-labeled CRH neurons, PI-stained cytoarchitecture and the merge of enlarged views of the area indicated by a red arrowhead and a white box in CEA in (c3). Scale bars: (A,B) 1 mm, (C) 1 mm, and (D) 30 μm. Secondary motor area (MOs), piriform area (PIR), nucleus accumbens (ACB), PVH, CEA, hippocampal formation (HPF), B, and IO.

Article Snippet: CRH-Cre transgenic mice ( ) and EYFP Cre reporter Ai3 mice ( ) were obtained from the Josh Huang Lab (CSHL, Cold Spring Harbor, NY, United States) and Jackson Laboratories (JAX Mice stock number: 007903), respectively.

Techniques: Labeling, Staining

Journal: Frontiers in Neuroanatomy

Article Title: A Quantitative Analysis of the Distribution of CRH Neurons in Whole Mouse Brain

doi: 10.3389/fnana.2017.00063

Figure Lengend Snippet: Locating EYFP-labeled CRH neurons in the whole CRH-IRES-Cre;Ai3 mouse brain. (A) Locating neurons in 100 μm typical image stacks, presented in maximum intensity projection. (B) Three-dimensional reconstruction and (C,D) projection views of the data cubes indicated as white boxes in (A) , respectively. Gray and green signals represent CRH neuronal somas. Red dots represent the recognized centers of CRH neurons. The white, red, blue and orange arrowheads indicated the axial-overlapping neurons, low intensity neurons, error recognition neurons and misidentification neurons, respectively. (E) Counting accuracy. Blue and orange represent the recall and precision ( n = 12), respectively. Each data cube was randomly selected at a size of 300 μm × 300 μm × 300 μm. Scale bars: (A) 1 mm and (C,D) 50 μm.

Article Snippet: CRH-Cre transgenic mice ( ) and EYFP Cre reporter Ai3 mice ( ) were obtained from the Josh Huang Lab (CSHL, Cold Spring Harbor, NY, United States) and Jackson Laboratories (JAX Mice stock number: 007903), respectively.

Techniques: Labeling

Journal: Frontiers in Neuroanatomy

Article Title: A Quantitative Analysis of the Distribution of CRH Neurons in Whole Mouse Brain

doi: 10.3389/fnana.2017.00063

Figure Lengend Snippet: The segmentation of anatomical regions. (A) A 10-μm maximum intensity projection of representative PI-stained coronal slices in the same mouse brain. The cyan lines represent the segmented results using the ANTS tool. (B) EYFP-labeled CRH neurons and PI-stained cytoarchitecture of the enlarged view of the area indicated by a green rectangular box in (A) . Green represents the 100 μm maximum intensity projection of EYFP-labeled CRH neurons. Red represents the 2 μm original data of PI-stained cytoarchitecture. White lines represent the laminated contours of the cortex. (C) Quantitative statistics of EYFP-labeled CRH neurons in different layers in MOp, MOs, VIS, AUD, SSp and SSs. Scale bar: (A) 1 mm, (B) 100 μm. Abbreviations: olfactory areas (OLF), pallidum (PAL), striatum (STR), hypothalamus (HY), cortical subplate (CTXsp), hippocampal formation (HPF), thalamus (TH), midbrain (MB), pons (P), medulla (MY), cerebellum (CB), primary motor area (MOp), secondary motor area (MOs), visual area (VIS), auditory area (AUD), primary somatosensory area (SSp), and supplemental somatosensory area (SSs).

Article Snippet: CRH-Cre transgenic mice ( ) and EYFP Cre reporter Ai3 mice ( ) were obtained from the Josh Huang Lab (CSHL, Cold Spring Harbor, NY, United States) and Jackson Laboratories (JAX Mice stock number: 007903), respectively.

Techniques: Staining, Labeling

Journal: Frontiers in Neuroanatomy

Article Title: A Quantitative Analysis of the Distribution of CRH Neurons in Whole Mouse Brain

doi: 10.3389/fnana.2017.00063

Figure Lengend Snippet: Quantitative statistics of the distribution of EYFP-labeled CRH neurons in the CRH-IRES-Cre;Ai3 mouse brain. (A) Recognized positions of EYFP-labeled neurons in a CRH-IRES-Cre;Ai3 mouse brain. Different color points represent the soma centers of CRH neurons in different regions. (B) Cell densities of EYFP-labeled CRH neurons in 12 brain regions. Color definitions are the same as those shown in (A) . (C) Cell density and nuclei distance of EYFP-labeled CRH neurons in the sub-regions of the above brain regions.

Article Snippet: CRH-Cre transgenic mice ( ) and EYFP Cre reporter Ai3 mice ( ) were obtained from the Josh Huang Lab (CSHL, Cold Spring Harbor, NY, United States) and Jackson Laboratories (JAX Mice stock number: 007903), respectively.

Techniques: Labeling

Journal: Frontiers in Neuroanatomy

Article Title: A Quantitative Analysis of the Distribution of CRH Neurons in Whole Mouse Brain

doi: 10.3389/fnana.2017.00063

Figure Lengend Snippet: Analyzing soma morphology of EYFP-labeled CRH neurons in eleven discrete brain areas. (A) EYFP-labeled CRH neurons in 12 discrete brain areas. Each data cube was randomly selected at the size of 200 μm × 200 μm × 200 μm. Scale bar: 50 μm. (B) Extracting the morphological features of CRH neuronal somas using PCA transformation. Two normalized main features were acquired after PCA on the six morphological parameters. The cyan lines surrounding the most concentrated neurons with similar soma morphology account for 80% of the total neurons in each region. (C) SDs of both main features in the main regions except CB. (D) An ANOVA was conducted to analyze both of the main features. The light and dark colors represent P < 0.05 and P ≥ 0.05, respectively. Primary somatosensory area (SSp), MOB, Ammon’s horn (CA), endopiriform nucleus, dorsal part (EPd), CEA, bed nuclei of the stria terminalis (BST), central medial nucleus of the thalamus (CM), PVH, periaqueductal gray (PAG), PG, IO, and hemispheric regions (HEM).

Article Snippet: CRH-Cre transgenic mice ( ) and EYFP Cre reporter Ai3 mice ( ) were obtained from the Josh Huang Lab (CSHL, Cold Spring Harbor, NY, United States) and Jackson Laboratories (JAX Mice stock number: 007903), respectively.

Techniques: Labeling, Transformation Assay